Coding
STATH(Stat

Part:BBa_K3736000

Designed by: Yi Hua Li   Group: iGEM21_NCTU_Formosa   (2021-08-19)
Revision as of 20:20, 21 October 2021 by Ihua li (Talk | contribs)


STATH(Statherin)
   A protein that promotes tooth remineralization and repairs decalcified enamel.
   Statherin is a protein in humans that is encoded by the STATH gene. It prevents the precipitation of calcium phosphate in saliva, maintaining a high calcium level in saliva available for remineralization of tooth enamel and high phosphate levels for buffering.

STATH Functional Test

   Carbon rods are processed to have small, low-volume pores that increase the surface area available for adsorption. We expected that both STATH and calcium could adsorb on the surface of carbon rods, so we designed a test to observe the surface of carbon rods soaked in different solutions under the microscope.

  We made four solutions configured to calcium ions saturated solutions by the calcium chloride solution, ddH2O, LB broth, DenTeeth, and E. coli with pSB1K3, in the tubes with the carbon rods stuck inside. After putting them into a 0°C refrigerator for 12 hours, the solutions would become calcium ions supersaturated solutions. In the DenTeeth, STATH would bind onto the surface of the carbon rod, preventing the calcium ions from binding onto it.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None