Composite
Urea Senso

Part:BBa_K3710004

Designed by: Sandra Lizeth Martinez Diaz, Erik Hanko and Katherine Baker   Group: iGEM21_Manchester   (2021-10-01)
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Urea Biosensor

This part harbours the urea biosensor composed of the urea-responsive transcriptional regulator UreR and the bidirectional promoter of the Proteus mirabilis urease operon PureD.

Proteus mirabilis encodes the transcriptional regulator UreR of the urease gene cluster. It belongs to the AraC/XylS family of transcriptional regulators. UreR upregulates the gene clusters’ subunit transcription in the presence of urea and recruits the accessory proteins (UreDABCEFG), forming an active urease [1].

Pathogenic bacteria produce virulence factors, such as ureases, to assist in their colonisation efforts. Consequently, these ureases hydrolyse urea into NH3 and Carbonic acid, which increase local pH and provide a processed nitrogen source for growth. These changes assist the bacteria, but also harm the host, causing mineral deposition on the urethral lumen resulting in urinary stones [2].

PureD is an inducible promoter that is activated by the UreR transcriptional regulator in the presence of urea. PureD is one of several promoters activated by the UreR transcriptional activator which share a consensus sequence of TA/GT/CA/TT/GC/TTA/TT/AATTG [3].

References:

[1] Poore, C. and Mobley, H. (2003) "Differential regulation of the Proteus mirabilis urease gene cluster by UreR and H-NS", Microbiology, 149(12), pp. 3383-3394.

[2] Dattelbaum, J. et al. (2003) "UreR, the Transcriptional Activator of the Proteus mirabilis Urease Gene Cluster, Is Required for Urease Activity and Virulence in Experimental Urinary Tract Infections", Infection and Immunity, 71(2), pp. 1026-1030.

[3]. Thomas V, Collins C. Identification of UreR binding sites in the Enterobacteriaceae plasmid-encoded and Proteus mirabilis urease gene operons. Molecular Microbiology. 1999;31(5):1417-1428.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1982
    Illegal AgeI site found at 2094
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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