Plasmid

Part:BBa_K3861005

Designed by: Kristin Funke   Group: iGEM21_Humboldt_Berlin   (2021-10-01)
Revision as of 20:12, 21 October 2021 by KristinHU (Talk | contribs)


pKF01

Introduction

iGEM Humboldt_Berlin 2021 standard backbone derived from pSB1C3. It contains the standard the high-copy-number ColE1/pMB1/pUC origin of replication. The chloramphenicol resistance cassette of pSB1C3 was replaced with the coding sequences of thyA (BBa_K3861003) and lldR (BBa_K3861004). The expression of thyA and lldR is driven by the synthetic constitutive promoter P_syn-thyA (BBa_K3861002) (Stringer et al., 2013). pKF01 still contains the binding sites for the standard sequencing/amplification primers for BioBrick parts, VF2 and VF. We used this backbone as part of the composite part / backbone BBa_K3861026.

Biology

pKF01
Fig. 1 - GFP expression in pSB1C3 (plate on top) and in pKF01 (plate below).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 997
    Illegal XhoI site found at 2804
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None