Plasmid
Part:BBa_K3861005
Designed by: Kristin Funke Group: iGEM21_Humboldt_Berlin (2021-10-01)
pKF01
Introduction
iGEM Humboldt_Berlin 2021 standard backbone derived from pSB1C3. It contains the standard the high-copy-number ColE1/pMB1/pUC origin of replication. The chloramphenicol resistance cassette of pSB1C3 was replaced with the coding sequences of thyA (BBa_K3861003) and lldR (BBa_K3861004). The expression of thyA and lldR is driven by the synthetic constitutive promoter P_syn-thyA (BBa_K3861002) (Stringer et al., 2013). pKF01 still contains the binding sites for the standard sequencing/amplification primers for BioBrick parts, VF2 and VF. We used this backbone as part of the composite part / backbone BBa_K3861026.
Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 997
Illegal XhoI site found at 2804 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |