Coding

Part:BBa_K3747008

Designed by: Riemer van der Vliet   Group: iGEM21_Wageningen_UR   (2021-10-21)
Revision as of 20:01, 21 October 2021 by Riemer (Talk | contribs)

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huHF scaffold


Apo form of human heavy-chain ferritin. Used as scaffold in Kim et al 2019 [1]. Here this part was used as scaffold for synthetic mimic of particulate methane monooxygenase.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 310
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 310
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 310
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 310
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Kim, H.J., Huh, J., Kwon, Y.W. et al. Biological conversion of methane to methanol through genetic reassembly of native catalytic domains. Nat Catal 2, 342–353 (2019). https://doi.org/10.1038/s41929-019-0255-1

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