Coding

Part:BBa_K3739027

Designed by: Ziyan Han   Group: iGEM21_XMU-China   (2021-09-08)
Revision as of 19:57, 21 October 2021 by Teslwang (Talk | contribs) (→‎Usage)

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LamB1-LC1KR2-LamB2-his

We anchor LCIKR-2 protein onto membranes through LamB to stick the engineered bacteria on the polypropylene with an improved solution. We use BBa_K3739057 to construct the expression system and anchor LCIKR-2 on the surface of VnDX.

Biology

LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCIKR-2, A mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly. LCIKR-2 is fused with LamB used as a sandwich-anchoring motif so that LCIKR-2 can be displayed on the surface of VnDX.

Usage

Here, we use BBa_3739027 to construct the expression system, which may achieve surface display of LCIKR-2 on VnDX to help the VnDX adhere to polypropylene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 612
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 760


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Categories
Parameters
None