Device

Part:BBa_K4041005

Designed by: Derek Zhang   Group: iGEM21_HKIS   (2021-10-21)
Revision as of 19:54, 21 October 2021 by Oranges (Talk | contribs)


TDH cas12a crRNA transcription device (crRNA1)

Template for T7 transcription of crRNA for Vibrio pathogenic gene marker. Need to be used with a XbaI overnight clean cut or PCR for the transcription to end at break point, to generate a short crRNA instead of transcription doesn't end. Designed on 100% conserved region.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None