Part:BBa_K3799006

R0010(plac promoter)+I746001(AIP1 generator - agrB + agrD with RBSes and terminator)

This part consist of the AIP generator(I746001) under standard IPTG inducible plac promoter(R0010) of Ecoli.It is used to express S aureus quorum sensing molecule,AIP-1 in Ecoli host. The biosynthesis of AIP requires AgrD, the peptide precursor of AIP, and the integral membrane endopeptidase AgrB.AIP-1 regulates the production of extracellular virulence factors using the agr quorum-sensing system by regulating P2 and P3 promoters.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

Quorum sensing in S. aureus is regulated by cyclic thiolactone peptides known as autoinducing peptides (AIPs). The extracellular AIP produced by neighbouring bacteria is sensed by a two-component system encoded in the accessory gene regulator (agr) locus. At high concentration of AIP-1, the cascade is activated, leading to the production of virulence factors including toxins, superantigens, and exo-enzymes.

The biosynthesis of AIP requires AgrD which encodes the peptide precursor of AIP, and an integral membrane endopeptidase AgrB. AgrD propeptide produced initially localizes to the cytoplasmic membrane using the N-terminal amphipathic leader and the AgrB endopeptidase activity removes the AgrD C-terminal tail.

Characterization