Part:BBa_K245114:Experience
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iGEM NCTU_Formosa(2021) |
Colony ResultWe successfully built DenTeeth with LL-37 . ModelThrough the modeling built with substituting parameters from published articles, the growth of E. coli and P. gingivalis under the effect of LL-37 would be inhibited significantly. However, the figure also shows that E. coli could still live with LL-37. Simply speaking, we successfully test the feasibility of DenTeeth and find that we can improve the DenTeeth with both biobrick design and efficiency optimization model. Because P. gingivalis was in the RG2, so we could not do the LL-37 functional test by inhibit the growth of P. gingivalis. After reading some related papers, we found that the killing rate of E. coli was similar to that of P. gingivalis [4]. Based on these data, we determined to make E. coli, DH5α with pET32A, as the bacteria killed by DenTeeth in the LL-37 functional test. Functional Test After finishing the design of DenTeeth, we wanted to know whether its inhibition ability can have a function, so we designed the following experiment.
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BBa_K245114 NCTU-Formosa(2021) Not understood UNIQ826d452e0ae36e63-partinfo-00000003-QINU