Coding

Part:BBa_K3739027

Designed by: Ziyan Han   Group: iGEM21_XMU-China   (2021-09-08)
Revision as of 19:19, 21 October 2021 by Teslwang (Talk | contribs) (→‎Usage and Biology)


LamB1-LC1KR2-LamB2-his

We anchor LCI-KR2 protein onto membranes through LamB to stick the engineered bacteria on the polypropylene with an improved solution. We use BBa_K3739057 to construct the expression system and anchor LCI-KR2 on the surface of VnDX.

Usage and Biology

LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. LCIKR-2, A mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly. LCIKR-2 is fused with LamB used as a sandwich-anchoring motif so that LCIKR-2 can be displayed on the surface of VnDX.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 612
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 760


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Categories
Parameters
None