Part:BBa_K4041004

Composite vibrio CDS template (TDH-vcgC-ctxA)

This is a non-coding part that consist of the TDH gene of the Vibrio Parahaemolyticus, vcgC gene of Vibrio vulnificus and the ctxA gene of Vibrio Cholerae. The genome template is used for cloning into a plasmid and contains conserved regions so teams could design detection kit based on the conserved regions.

The genes is one of the central and important gene that makes the Vibrios pathogenic. While it is important for its cytotoxicity, it itself could be served as a biomarker for the strains.

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Composite Part List:
Link:	Name and Function:
<a href="https://parts.igem.org/Part:BBa_K4041015">https://parts.igem.org/Part:BBa_K4041015</a>	Expression Cassette for pET-Yafl-Ulp1
<a href="https://parts.igem.org/Part:BBa_K4041016">https://parts.igem.org/Part:BBa_K4041016</a>	Expression Cassette for pET-LO-SUMO
<a href="https://parts.igem.org/Part:BBa_K4041017">https://parts.igem.org/Part:BBa_K4041017</a>	Expression Cassette for pET-LO-SUMO-AMP-Tachy Original
<a href="https://parts.igem.org/Part:BBa_K4041018">https://parts.igem.org/Part:BBa_K4041018</a>	Expression Cassette for pET-LO-SUMO-AMP1-Tachy 930
<a href="https://parts.igem.org/Part:BBa_K4041019">https://parts.igem.org/Part:BBa_K4041019</a>	Expression Cassette for pET-LO-SUMO-AMP1-Tachy 4303
<a href="https://parts.igem.org/Part:BBa_K4041020">https://parts.igem.org/Part:BBa_K4041020</a>	Expression Cassette for pET-LO-SUMO-AMP1-Tachy 5235
<a href="https://parts.igem.org/Part:BBa_K4041004">https://parts.igem.org/Part:BBa_K4041004</a>	Composite vibrio CDS template (TDH-vcgC-ctxA)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 295
  Illegal BsaI.rc site found at 477