Composite

Part:BBa_K3739050

Designed by: Ziyan Han   Group: iGEM21_XMU-China   (2021-09-09)
Revision as of 18:21, 21 October 2021 by Teslwang (Talk | contribs) (→‎1. Identification)


J23100-B0030-LC1KR-2-GFP-B0010

LCIKR-2 is a mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly. We use BBa_K3739050 to verify its capability by purification the protein.

Biology

LCIKR-2 is a mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly.. GFP is green fluorescent protein from jellyfish Aequorea Victoria, which has been widely used as reporter for decades. GFP is fused at C terminal with LCIKR-2 so that the expression of LCIKR-2 will be reported.

Characterization

1. Identification

After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure 1.

Fig. 1. Gene circuit and agarose gel electrophoresis. (A) Gene circuit of J23100-B0030-LC1KR-2-GFP-B0010 (BBa_K3739050). (B) Target bands of LCIKR-2-GFP (black arrow, 1200 bp).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 263


[edit]
Categories
Parameters
None