Part:BBa_K3777029

luxR-Plux-dCas9-mphA

Basic biosensor device for tetracycline detection.

Usage and Biology
The genetic circuit was composed of a coding sequence of tetracycline repressor which was inserted into an expression vectors with a consitive promoter(BBa_J23114) and RBS(BBa_K3777030), as well as 3WJdB(BBa_K3777000) under the control of T7 promoter (BBa_K3777006). The terminator we used were BBa_B0010 and BBa_M36305.(Fig 1)
When tet was absent, TetR would bind to the inducible promoter（PI）and prevent RNA polymerase from initiating transcription, thus repressing the expression of reporter gene. If tet was present, TetR would no longer able to bind to the promoter, resulting in the expression of reporter gene.
We expressed this circuit in the E. coli BL21(DE3) cells for tetracycline detection. Thus we could roughly deduce the concentration of the antibiotics in the sample according to the fluorescence intensity.

Referernce:Miller Corwin A,Ho Joanne M,Parks Sydney E,Bennett Matthew R. Macrolide Biosensor Optimization through Cellular Substrate Sequestration.[J]. ACS synthetic biology,2021,10(2)
Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 2204
  Illegal NheI site found at 5364
  Illegal NheI site found at 5387
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 4483
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 5951
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1022