Composite
Bst-E.ssb

Part:BBa_K3790152

Designed by: Guanqiao Chi   Group: iGEM21_Fudan   (2021-10-16)
Revision as of 17:51, 21 October 2021 by TiTong Fudan (Talk | contribs) (Usage and Biology)


Bst-(G2S)3-E.ssb


Introduction

2021 Fudan

The fusion protein obtained by linking the C-terminus of Bst to the N-terminal of E.ssb via a (G2S)3 linker.

Experimental Results

The length of xxxx DNA was xxx bp, which is approximately xxx bp after adding homology arms to both ends for PCR cloning. We isolated the DNA of interest by gel extraction for subsequent reactions.

Figure 2. Assembled DNA binding proteins, albA1, S.ssb, E.ssb. The first lane was loaded with DNA ladder, sizes were marked on the image. The brightest band of 750 bp was about 100 ng, and other bands about 50 ng. Lanes with correct sized amplified DNA were labeled. After PCR cloning, several bacterial clones were picked, grew into cultures and sent for Sanger sequencing. Then, we verified the sequencing results, and used the correct ones for further experiments.


Reference

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
Parameters
None