Composite
Part:BBa_K3783018:Design
Designed by: Satvik Kethireddy Group: iGEM21_OhioState (2021-10-21)
pFraB + LpxERF
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 880
Illegal PstI site found at 1536 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 880
Illegal PstI site found at 1536 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 880
Illegal PstI site found at 1536 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 880
Illegal PstI site found at 1536 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1970
Illegal SapI.rc site found at 1103
Design Notes
Scarless fusion
Source
Salmonella Enterica