Coding
Part:BBa_K3783015:Design
Designed by: Satvik Kethireddy Group: iGEM21_OhioState (2021-10-21)
LpxERF
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 768
Illegal PstI site found at 1424 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 768
Illegal PstI site found at 1424 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 768
Illegal PstI site found at 1424 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 768
Illegal PstI site found at 1424 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1858
Illegal SapI.rc site found at 991
Design Notes
Native Salmonella RBS and stop codons between CDS of LpxE and LpxR; and LpxR and LpxF
Source
Salmonella Enterica