Coding

Part:BBa_K3739020

Designed by: Han Ziyan   Group: iGEM21_XMU-China   (2021-09-08)
Revision as of 17:37, 21 October 2021 by Teslwang (Talk | contribs) (→‎Characterization)


LCIKR2-GFP

LCI-KR2 is a mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly. We use BBa_K3739050 to verify its capability by purification the protein.

Biology

LCI-KR2 is a mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly.. GFP is green fluorescent protein from jellyfish Aequorea Victoria, which has been widely used as reporter for decades. GFP is fused at C terminal with LCI-KR2 so that the expression of LCI-KR2 will be reported.

Characterization

1. Identification

After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure 1. 

 Fig. 3. Gene circuit involved in protein expression and AGE analysis of string a, Gene circuit was constructed by BBa_K3739021 to express LCIKR-2-GFP. b, Target bands of LCIKR-2-GFP (black arrow, 1200 bp).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 199


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Categories
Parameters
None