Part:BBa_K4080012
Sec system (PhoA), TEV protease with T7 expression
T7 expression systems are capable of producing a wide range of proteins in E. coli. TEV protease is a display protein of interest used to measure the yield of protein produced by the system. This composite is one of two that will be working to generate a TEV protease. This composite is expressed extracellularly through the Sec pathway. Characterization of two expression systems (between universal and T7) is measured to ensure great efficiency of expression. Characterization of the overall system contributes to improving the new era of protein overexpression.
Introduction
 UBrawijaya iGEM 2021 project CHOP systemis an outer membrane vesicle based protein overexpression system that includes three goals towards producing a blueprint for an ideal protein production system. This project can be customized because the system can be adapted with various proteins of interest, the overexpression system that can increase protein yield, and the clean harvest with simplified purification steps that is equipped with a protease enzyme expression mechanism in the concentrated system - from vesicles and cell pellets. |
This composite aims to expressed TEV protease through T7 promoter and translocates TEV protease across the cell membrane and out of the cell through the Sec pathway under PhoA signal sequence
Result
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |