Part:BBa_K3802000:Design
PgsA-MlrA fusion protein
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1792
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 827
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 813
Illegal NgoMIV site found at 2323 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 304
Illegal BsaI.rc site found at 2160
Design Notes
PgsA is not orginally from E.coli, but rather from Bacillus subtilis. Therefore, we had to codon optimize the entire part to be compatible with E.coli.
Source
This part is a composite of multiple parts from the registry. It was synthesized by a gene synthesizing company (IDT).
References
Dziga, D., Wladyka, B., Zielińska, G., Meriluoto, J., & Wasylewski, M. (2012). Heterologous expression and characterisation of microcystinase. Toxicon, 59(5), 578–586. https://doi.org/doi.org/10.1016/j.toxicon.2012.01.001
Maqsood, I., Shi, W., Wang, L., Wang, X., Han, B., Zhao, H., Nadeem, A. M., Moshin, B. S., Saima, K., Jamal, S. S., Din, M. F., Xu, Y., Tang, L., & Li, Y. (2018). Immunogenicity and Protective efficacy of orally administered recombinant Lactobacillus Plantarum expressing VP2 protein against IBDV in chicken. Journal of Applied Microbiology, 125(6), 1670–1681. https://doi.org/10.1111/jam.14073
Narita, J., Okano, K., Tateno, T., Tanino, T., Sewaki, T., Sung, M.-H., Fukuda, H., & Kondo, A. (2005). Display of active enzymes on the cell surface of Escherichia coli using PgsA anchor protein and their application to bioconversion. Applied Microbiology and Biotechnology, 70(5), 564–572. https://doi.org/10.1007/s00253-005-0111-x
Zhang, Y., Dong, W., Lv, Z., Liu, J., Zhang, W., Zhou, J., Xin, F., Ma, J., & Jiang, M. (2018). Surface display of bacterial Laccase CotA on Escherichia coli cells and its application in Industrial Dye Decolorization. Molecular Biotechnology, 60(9), 681–689. https://doi.org/10.1007/s12033-018-0103-6