Coding
E.ssb
Part:BBa_K3790006
Designed by: Guanqiao Chi Group: iGEM21_Fudan (2021-10-01)
E.ssb
Introduction
E.ssb is a single-stranded binding protein from Escherichia coli str. K-12 substr. MG1655[1]. We wanted to test whether the single-stranded binding protein could enhance the enzymatic activity of Bst Pol using the change in the activity of the fusion protein composed of it and Bst Pol.
Usage and Biology
Experimental Results
The length of albA1 DNA was 288 bp, which is approximately 300 bp after adding homology arms to both ends for PCR cloning. We isolated the DNA of interest by gel extraction for subsequent reactions.
Reference
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//cds
None |