Regulatory

Part:BBa_K239002:Design

Designed by: Axel Nystrom   Group: iGEM09_UCL_London   (2009-06-22)
Revision as of 09:37, 29 June 2009 by Axelnystrom (Talk | contribs) (→‎Source)

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HypB promoter, activated by Fnr during oxygen deficiency


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first Fnr binding site and ends 37 pb after second Fnr binding site.


Source

Genomic sequence from biocyc, Escherichia coli K-12. Native unmodified sequence. Reference: FNR-mediated regulation of hyp expression in Escherichia coli. Sarah L. Messenger, Jeffrey Green, 2003. FEMS Microbiology Letters 228 (2003) 81-86

References