Regulatory

Part:BBa_K3905004

Designed by: Peter Heywood, Harry Weiniger   Group: iGEM21_City_of_London_UK   (2021-09-09)
Revision as of 16:18, 21 October 2021 by Hweiniger (Talk | contribs)


gen2 517-5p Toehold Switch

This Toehold Switch is activated by hsa-MiRNA 517-5p. The micro RNA 517-5p alone acts as the trigger for the toehold switch and causes the riboregulator to unfold, exposing the RBS and start codon. Placing a reporter protein downstream of this part will mean that when miRNA 517-5p is present in high enough quantities, the protein will be translated and the reporter protein can be observed.

Labelled toehold switch in its 'off' state.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 15
    Illegal XbaI site found at 53
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 15
    Illegal XbaI site found at 53
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 15
    Illegal XbaI site found at 53
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None