Coding

Part:BBa_K4060505:Design

Designed by: Cheng-Ju Lu   Group: iGEM21_NYCU-Taipei   (2021-10-21)
Revision as of 16:06, 21 October 2021 by Registry (Talk | contribs)

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BphP1 gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 416
    Illegal XhoI site found at 2166
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
    Illegal NgoMIV site found at 79
    Illegal NgoMIV site found at 367
    Illegal NgoMIV site found at 402
    Illegal NgoMIV site found at 1650
    Illegal NgoMIV site found at 2152
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1207
    Illegal BsaI.rc site found at 2053


Design Notes

The construction of this part is according to igem assembly protocol.


Source

The source of the gene is from Addgene plasmid pKA-207I10.

References