Coding

Part:BBa_K3788011

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-27)
Revision as of 15:52, 21 October 2021 by Liam Z (Talk | contribs)

(diff) ↠Older revision | Latest revision (diff) | Newer revision → (diff)

NOTOC Aida-I (D33:N) T5SS


BBa_K3788011 is coding for AidaA-I (D>N). It is an autotransporter from the T5SS; it allow the addressing to the extracellular medium, with a mutation that permit to have no protein cleavage, and keep it the passenger protein attached to the extracellular membrane.

The sequence is coding for the beta-barrel sequence that forms a pore in the external membrane and a linker that allows a passenger protein to go through the beta barrel to join the extracellular medium.

Wild-Type Aida-I has an autolytic activity that allows the passenger protein cleavage from the rest of the complex so it is released in the extracellular medium, but we introduced D:33N substitution the the cleavage site is not recognized anymore and the passenger protein remains attached to the cell.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1084
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None