Composite

Part:BBa_K3747606

Designed by: Sanne van Doorn   Group: iGEM21_Wageningen_UR   (2021-10-21)
Revision as of 15:02, 21 October 2021 by Sanvdoorn (Talk | contribs)


Denitrification BAC

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Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1348
    Illegal EcoRI site found at 1957
    Illegal EcoRI site found at 3310
    Illegal EcoRI site found at 16935
    Illegal EcoRI site found at 17859
    Illegal EcoRI site found at 24833
    Illegal SpeI site found at 3261
    Illegal PstI site found at 5615
    Illegal PstI site found at 5693
    Illegal PstI site found at 12267
    Illegal PstI site found at 13293
    Illegal PstI site found at 13539
    Illegal PstI site found at 14118
    Illegal PstI site found at 14805
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1348
    Illegal EcoRI site found at 1957
    Illegal EcoRI site found at 3310
    Illegal EcoRI site found at 16935
    Illegal EcoRI site found at 17859
    Illegal EcoRI site found at 24833
    Illegal NheI site found at 7003
    Illegal NheI site found at 25343
    Illegal SpeI site found at 3261
    Illegal PstI site found at 5615
    Illegal PstI site found at 5693
    Illegal PstI site found at 12267
    Illegal PstI site found at 13293
    Illegal PstI site found at 13539
    Illegal PstI site found at 14118
    Illegal PstI site found at 14805
    Illegal NotI site found at 30477
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1348
    Illegal EcoRI site found at 1957
    Illegal EcoRI site found at 3310
    Illegal EcoRI site found at 16935
    Illegal EcoRI site found at 17859
    Illegal EcoRI site found at 24833
    Illegal BglII site found at 813
    Illegal BglII site found at 2951
    Illegal BglII site found at 4947
    Illegal BglII site found at 5346
    Illegal BglII site found at 5763
    Illegal BglII site found at 7819
    Illegal BglII site found at 10451
    Illegal BamHI site found at 22426
    Illegal XhoI site found at 1529
    Illegal XhoI site found at 2632
    Illegal XhoI site found at 13020
    Illegal XhoI site found at 15918
    Illegal XhoI site found at 17157
    Illegal XhoI site found at 24137
    Illegal XhoI site found at 24902
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1348
    Illegal EcoRI site found at 1957
    Illegal EcoRI site found at 3310
    Illegal EcoRI site found at 16935
    Illegal EcoRI site found at 17859
    Illegal EcoRI site found at 24833
    Illegal SpeI site found at 3261
    Illegal PstI site found at 5615
    Illegal PstI site found at 5693
    Illegal PstI site found at 12267
    Illegal PstI site found at 13293
    Illegal PstI site found at 13539
    Illegal PstI site found at 14118
    Illegal PstI site found at 14805
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1348
    Illegal EcoRI site found at 1957
    Illegal EcoRI site found at 3310
    Illegal EcoRI site found at 16935
    Illegal EcoRI site found at 17859
    Illegal EcoRI site found at 24833
    Illegal SpeI site found at 3261
    Illegal PstI site found at 5615
    Illegal PstI site found at 5693
    Illegal PstI site found at 12267
    Illegal PstI site found at 13293
    Illegal PstI site found at 13539
    Illegal PstI site found at 14118
    Illegal PstI site found at 14805
    Illegal NgoMIV site found at 1285
    Illegal NgoMIV site found at 2306
    Illegal NgoMIV site found at 3076
    Illegal NgoMIV site found at 3923
    Illegal NgoMIV site found at 4225
    Illegal NgoMIV site found at 4715
    Illegal NgoMIV site found at 5252
    Illegal AgeI site found at 1768
    Illegal AgeI site found at 3772
    Illegal AgeI site found at 6314
    Illegal AgeI site found at 6860
    Illegal AgeI site found at 10390
    Illegal AgeI site found at 13430
    Illegal AgeI site found at 19220
  • 1000
    COMPATIBLE WITH RFC[1000]

The Denitrification BAC encodes for the complete denitrification machinery from P. stutzeri JM300. The system is flanked by two incompatible lox sites to ensure genomic integration into P. putida EM42. Integration can be realized through the Cre-lox mediated recombination.


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