Part:BBa_K3924033

LAP

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Profile

Name: LAP
Base Pairs: 2925bp
Origin: Listeria innocua, synthetic
Properties: Listeria adhesion protein (LAP) from a non-pathogenic Listeria (L. innocua)

Usage and Biology

We bioengineered Lactococcus lactis to express the Listeria adhesion protein (LAP) from a non-pathogenic Listeria (L. innocua). L. lactis colonize the intestine, reduce Lm mucosal colonization and systemic dissemination, and protect mice from lethal infection. L. lactis expressing LAP has been reported to competitively exclude Listeria(Lm) by occupying the surface presented LAP receptor, and can ameliorate the Lm-induced intestinal barrier dysfunction by blocking the nuclear factor-κB and myosin light chain kinase-mediated redistribution of the major epithelial junctional proteins. ^[1]

Design and Construction

As our engineered probiotics are to be used in IBD treatment as prescribed drugs instead of dietary supplements, we deem it unnecessary to incorporate conditional promoters into our design. We therefore use the constitutive expression plasmid pMG36e as the backbone. Also, being a transfer plasmid, pMG36e can replicate in both E.coli and L.lactis, which saved us a lot of trouble in transformation.
center|600px|thumb|Figure 1: The design of LAP

Reference

[1] Drolia, R., Amalaradjou, M. A. R., Ryan, V., Tenguria, S., Liu, D., Bai, X., ... & Bhunia, A. K. (2020). Receptor-targeted engineered probiotics mitigate lethal Listeria infection. Nature communications, 11(1), 1-23.