Composite
Part:BBa_K4046920:Design
Designed by: Haipei Yao Group: iGEM21_Duke (2021-10-21)
CMV - BS #1 - BS #1 - Kozak - mCherry - bghA
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1038
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1038
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 614
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1038
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1038
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
tdTomato is the modified product of a gene originally from mushroom corals in the Discosoma family. The gene was obtained through PCR of a commercially available plasmid pCRISPR-HOT_tdTomato (Addgene, 138567). In normal function, tdTomato is a red fluorescence protein.
Source
This gene was synthesized commercially from the published sequence (IDT). The DhdR gene is a transcriptional repression factor that is derived from the bacteria Achromobacter denitrificans. The dhdO binding sites were designed and modified to improve binding behavior of the DhdR protein to the sequence.