Coding

Part:BBa_K3982001:Design

Designed by: Akankshya Ramkrishna Sahu   Group: iGEM21_IISER_Berhampur   (2021-10-01)
Revision as of 14:50, 21 October 2021 by Akankshya16 (Talk | contribs) (→‎Source)

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Cas14a1 expression gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1357
    Illegal PstI site found at 147
    Illegal PstI site found at 313
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1357
    Illegal PstI site found at 147
    Illegal PstI site found at 313
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1357
    Illegal BamHI site found at 951
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1357
    Illegal PstI site found at 147
    Illegal PstI site found at 313
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1357
    Illegal PstI site found at 147
    Illegal PstI site found at 313
    Illegal AgeI site found at 607
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It contains EcoR1 and Pst1 sites. To clone or excise this part, preferably use XBa1 and Spe1 Restriction Enzymes.


Source

Addgene plasmid # 112502