Composite

Part:BBa_K4015017

Designed by: Andrew Peng Yi   Group: iGEM21_KEYSTONE   (2021-10-21)
Revision as of 14:45, 21 October 2021 by Registry (Talk | contribs)

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CotA-NusA-Lcp1VH2-HlyA

CotA-NusA-Lcp-HlyA is composed of four basic parts, CotA, NusA, Lcp, and HlyA. The composition enables the secretion of Lcp with enhanced solubility and expression rate, with clear indication.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4440
    Illegal XhoI site found at 5070
    Illegal XhoI site found at 6595
    Illegal XhoI site found at 6749
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4689
    Illegal NgoMIV site found at 5047
    Illegal NgoMIV site found at 6241
    Illegal NgoMIV site found at 6563
    Illegal AgeI site found at 2713
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4674
    Illegal BsaI.rc site found at 186
    Illegal BsaI.rc site found at 1631
    Illegal BsaI.rc site found at 1849
    Illegal BsaI.rc site found at 6144
    Illegal SapI.rc site found at 4662


[edit]
Categories
Parameters
None