Part:BBa_K3764001
Module for generation of a T-vector with mRFP1 based selection
This part is a modified version of part BBa_K3764000. It contains the same specialized module for the generation of a T-vector upon Eam1105I (AhdI) digestion but here we integrated it into the ORF of an mRFP1 protein instead of using LacZalfa fragment. With this version, one can use the color-based selection option without adding IPTG, X-Gal and/or using specialized E. coli strains for cloning.
Once integrated into a BioBrick vector it will transform the plasmid into a TA cloning compatible one. PLEASE BEFORE USE CHECK IF THE BACKBONE CONTAINS A Eam1105I (AhdI) RECOGNITION SITE. If this is the case, all such sites should be mutagenized before obtaining a functional T-vector.
This part has no promoter and RBS module! You should add one if you like to use the color-based selection.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 43
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 51
Illegal BamHI site found at 160 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 750
Illegal AgeI site found at 862 - 1000COMPATIBLE WITH RFC[1000]
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