Part:BBa_K3764000
Module for generation of a T-vector
This part contains a specialized module for the generation of a T-vector upon Eam1105I (AhdI) digestion. Moreover, this part also supports the blue-white screening procedure available for vectors like pUC19 and pBluescript. Once integrated into a BioBrick vector it will transform the corresponding plasmid into a TA cloning compatible one. PLEASE BEFORE USE CHECK IF THE SELECTED VECTOR BACKBONE CONTAINS A Eam1105I (AhdI) RECOGNITION SITE. If this is the case, all such sites should be mutagenized before obtaining a functional T-vector.
This part has no promoter and RBS module! You should add one if you want to use the blue-white screening procedure with your newly generated T-vector.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 341
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 335
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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