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Part:BBa_K3764000

Designed by: Slavil Peykov   Group: iGEM21_Bulgaria   (2021-10-21)
Revision as of 14:21, 21 October 2021 by Slavil (Talk | contribs)


Module for generation of a T-vector

This part contains a specialized module for the generation of a T-vector upon Eam1105I (AhdI) digestion. Moreover, this part also supports the blue-white screening procedure available for vectors like pUC19 and pBluescript. Once integrated into a BioBrick vector it will transform the corresponding plasmid into a TA cloning compatible one. PLEASE BEFORE USE CHECK IF THE SELECTED VECTOR BACKBONE CONTAINS A Eam1105I (AhdI) RECOGNITION SITE. If this is the case, all such sites should be mutagenized before obtaining a functional T-vector.

This part has no promoter! You should add one if you want to use the blue-white screening procedure with your newly generated T-vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 341
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 335
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None