Composite
Part:BBa_K4015013
Designed by: Andrew Peng Yi Group: iGEM21_KEYSTONE (2021-10-21)
CotA-Lcp1VH2-HlyA
CotA-Lcp-HlyA is composed of three basic parts, Lcp1VH2, HlyA and CotA. HlyA and Lcp are connected by Gibson assembly in order to allow Lcp to be secreted outside the cell through HlyA,and assembling CotA on the composite part can help to indicate the secretion.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 2058
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1677
Illegal NgoMIV site found at 2035
Illegal AgeI site found at 1348 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1662
Illegal SapI.rc site found at 286
Illegal SapI.rc site found at 1650
[edit]
Categories
Parameters
None |