Composite

Part:BBa_K4015013

Designed by: Andrew Peng Yi   Group: iGEM21_KEYSTONE   (2021-10-21)
Revision as of 14:16, 21 October 2021 by Registry (Talk | contribs)

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CotA-Lcp1VH2-HlyA

CotA-Lcp-HlyA is composed of three basic parts, Lcp1VH2, HlyA and CotA. HlyA and Lcp are connected by Gibson assembly in order to allow Lcp to be secreted outside the cell through HlyA,and assembling CotA on the composite part can help to indicate the secretion.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2058
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1677
    Illegal NgoMIV site found at 2035
    Illegal AgeI site found at 1348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1662
    Illegal SapI.rc site found at 286
    Illegal SapI.rc site found at 1650


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Categories
Parameters
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