Terminator
Part:BBa_K4012021
Designed by: TIANLANG CHEN Group: iGEM21_AISSU_Union (2021-10-11)
Revision as of 14:13, 21 October 2021 by Supernova-Blaze (Talk | contribs)
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tENO2
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Secondary Structure
Measurement
- [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
Obtaining the fragment and BsaI digested verification
We construct tENO2 with vector type8-pSB1K3-GFP and proceed digested verification using BsaI. According to Fig.1, we obtain two clear bands after BsaI digested, the length of the vector(1622bp) and the inserted fragments tENO2(238bp) matched with previous expectations by compared with Marker MK8000 ladder, confirming the successful assembly of Toolkit plasmids and availability in subsequent construction.
tENO2 in Level1 plasmid assembly
. The initiation of the CsF3H sequence is done by promoter pTEF1, with termination done by tENO2. The sequence ConL2 and ConR3 are connector sequences within the Level 1 plasmid assembly. Furthermore, typr9-KVF and type9-VR are imposed to enact selection through colonies PCR, results shown in Fig.2 The band length 2500bp match with expectations. The sequence analysis results show no significant mutations or deletions, representing the success of the assembly.tENO2 in Level2 plasmid assembly
tENO2 is also involved in assembly of synthesis pathway of naringenin, shown by Fig.3.
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Parameters
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