Part:BBa_K4080010
amilCP-eCPX fusion with T7 expression
T7 expression systems are capable of producing a wide range of proteins in E. coli. Improvements to overexpression with clean harvest can be done under T7 control and using eCPX as an outer membrane. amilCP is a display protein of interest used to measure the yield of protein produced by the system. This composite is one of two that will be working to generate a CHOP system. Characterization of two expression systems (between T7 and non T7) is measured to select the best blueprint for the CHOP system. Characterization of the overall system contributes to improving the new era of protein overexpression.
Introduction
 UBrawijaya iGEM 2021 project CHOP systemis an outer membrane vesicle based protein overexpression system that includes three goals towards producing a blueprint for an ideal protein production system. This project can be customized because the system can be adapted with various proteins of interest, the overexpression system that can increase protein yield, and the clean harvest with simplified purification steps that is equipped with a protease enzyme expression mechanism in the concentrated system - from vesicles and cell pellets. |
This composite aims to know the feasibility of using the T7 expression system for overexpressing the pigmented protein known as chromoprotein (amilCP), and by utilizing the terminal display scaffold eCPX will be localized on the outer membrane of the E.coli host.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |