Regulatory

Part:BBa_K3814002

Designed by: Simon Tang   Group: iGEM21_Sydney_Australia   (2021-10-01)
Revision as of 14:01, 21 October 2021 by Simontang (Talk | contribs)


Psal1 (PsalTTC, 100% Activity)

Psal promoter with adjustments to have tighter repression without salicylate and higher expression when fully induced.

Promoter "-35" "-10"
Psal TTATCA TATCGT
PsalTTC TTATTT TACCGT


This example showcases a variant that yields 34% expression levels of WT Psal promoters. This is described more indepth in the design section.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 68
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None