Coding

Part:BBa_K3863005

Designed by: LEONG WENG I   Group: iGEM21_PuiChing_Macau   (2021-09-26)
Revision as of 13:52, 21 October 2021 by Alisaleong (Talk | contribs)

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fabB-his-ldhA-flag

This is a composite part designed for the acid tolerant system. FabB: coding sequence for 3-oxoacyl-[acyl-carrier-protein] synthase from Escherichia coli (strain K12). (reference: NCBI locus tag - b2323)[1] ldhA: Lactate dehydrogenase A (ldhA) is an enzyme catalyzing the synthesis of lactate from pyruvate. The protein sequence in this part is based on the K2588009 sequence Hamburg 2018.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1240
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 991
    Illegal AgeI site found at 1841
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 571
    Illegal SapI.rc site found at 721
    Illegal SapI.rc site found at 1084
    Illegal SapI.rc site found at 1571

Reference

[1]https://www.ncbi.nlm.nih.gov/gene/946799


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