Part:BBa_K3720003

Firefly luciferase with pCooM, RBS (weak), and double terminator

When used in combination with CooA, it is possible to create a light emitting mechanism that reacts with carbon monoxide. Culture pBEST incorporating this part in LD medium and transfer 20㎕ to a 1.5-ml tube. Then, 0.5㎕ of D-luciferin was added, CO was injected, and luminescence was measured. Quantitative measurements were not possible because the laboratory facilities did not allow us to prepare a luminometer. As shown in the photo, the pipette which carbon monoxide was injected showed a change in color.

figure.1 Color changes in bacterial culture
Left: LB medium+luciferin Right: LB medium+luciferin+CO gas

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 900