Regulatory

Part:BBa_K239001

Designed by: Axel Nystrom   Group: iGEM09_UCL_London   (2009-06-22)
Revision as of 11:28, 22 June 2009 by Registry (Talk | contribs)

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Spy promoter, activated by Phosporylated CpxR, BaeR and Sigma70

Sequence contains 2 phosphorylated BaeR binding sites, 1 phosphorylated CpxR binding site and transcription is initiated by sigma factor 70. Function: Can detect various periplasmic stresses. Area of application: Hopefully it can be used to detect presence of relevant unfolded proteins in the periplasmic space, to detect shear stress or other so far undefined relevant stress.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None