Regulatory

Part:BBa_K239000:Design

Designed by: Axel Nystrom   Group: iGEM09_UCL_London   (2009-06-20)
Revision as of 09:40, 21 June 2009 by Axelnystrom (Talk | contribs) (→‎Design Notes)

DegP promoter, activated by Phosporylated CpxR and SigmaE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. Only SpeI restriction site had to be removed. Some uncertainty about where to start and stop in the sequence. It starts 3 bp before the first CpxR binding site and ends 6 pb after the -10 box.

Source

Genomic sequence from biocyc, Escherichia coli K-12. Sequence modified to remove Spe1 restriction site.

References