Coding

Part:BBa_K4011006

Designed by: Aaron Zhexuan Zhang   Group: iGEM21_LINKS_China   (2021-10-21)
Revision as of 12:36, 21 October 2021 by Registry (Talk | contribs)

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AeAT9

AeAT9 is an alcohol acyltransferase, and is responsible for the production of ethyl-acetate from acetyl-CoA and ethanol in our fermentation process. We will express AeAT9 in yeast cells to synthesize ethyl-acetate from acetyl-CoA and ethanol, the second step into our pathway to turn acetate and ethanol into ethyl-acetate. We hope to give our yeast culture a fruit smell. This part will also be used to construct yeast expression plasmids through golden gate assembly into BBa_K4011017 and BBa_K4011019.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 199
    Illegal BglII site found at 817
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 226
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1064


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