![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4011006
Designed by: Aaron Zhexuan Zhang Group: iGEM21_LINKS_China (2021-10-21)
AeAT9
AeAT9 is an alcohol acyltransferase, and is responsible for the production of ethyl-acetate from acetyl-CoA and ethanol in our fermentation process. We will express AeAT9 in yeast cells to synthesize ethyl-acetate from acetyl-CoA and ethanol, the second step into our pathway to turn acetate and ethanol into ethyl-acetate. We hope to give our yeast culture a fruit smell. This part will also be used to construct yeast expression plasmids through golden gate assembly into BBa_K4011017 and BBa_K4011019.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 199
Illegal BglII site found at 817 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 226
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1064
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Categories
Parameters
None |