DNA
Stop5

Part:BBa_K3714016

Designed by: Yuxin Lu   Group: iGEM21_SJTU-BioX-Shanghai   (2021-09-30)
Revision as of 12:10, 21 October 2021 by Grus (Talk | contribs)


Stop oligo

An oligonucleotide designed for inhibit aptazyme activity temporarily by toehold meditated strand displacing (TMSD) reaction. It will denature an aptazyme (BBa_K3714010) by hybridization. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


K3714016.png
Figure.1 | Verification of stop oligo. The aptazyme was produced by a T7 in vitro transcription system. Gardiquimod is an inhibitor of the cleavage activity. The product was run on a denatured PAGE.


[edit]
Categories
Parameters
None