Composite

Part:BBa_K4011019

Designed by: Xiaochong Ma   Group: iGEM21_LINKS_China   (2021-10-21)
Revision as of 12:08, 21 October 2021 by Registry (Talk | contribs)

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pTALEsp2-TnaA-FL-FMO-B0015

pTALEsp2-TnaA-FL-FMO is an expression cassette in E. coli expressing TnaA-FL-FMO BBa_K4011004 used to convert 6-Halogen-Trpytophan (6-X-Trp) into di-halogenated indigoid dyes such as tyrian purple. pTALEsp2 No part name specified with partinfo tag. is an constitutive promoter suited to all strains of E. coli. B0015 BBa_B0015 is a strong terminator. This is a part in a part collection where we enable the production of indigo, tyrian purple, and related dyes from tryptophan in E. coli.

The part collection includes: Parts expressing Fre-SttH to convert Trp to 6-X-Trp. BBa_K4011003 and BBa_K4011012. Parts expressing fusion protein TnaA-FMO to convert 6-X-Trp into indigoid dyes. BBa_K4011004, BBa_K4011005, BBa_K4011013, BBa_K4011014, BBa_K4011015, and BBa_K4011019.

Our part collection can be used to help and inspire future teams to design and perfect different indigoid dye production pathways in E. coli, adding to the collection.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1513
    Illegal NheI site found at 1921
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 113
    Illegal BamHI site found at 5978
    Illegal XhoI site found at 2152
    Illegal XhoI site found at 2692
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 747
    Illegal AgeI site found at 849
    Illegal AgeI site found at 1767
    Illegal AgeI site found at 2277
    Illegal AgeI site found at 4385
  • 1000
    COMPATIBLE WITH RFC[1000]


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