Part:BBa_K4011019
pTALEsp2-TnaA-FL-FMO-B0015
pTALEsp2-TnaA-FL-FMO is an expression cassette in E. coli expressing TnaA-FL-FMO BBa_K4011004 used to convert 6-Halogen-Trpytophan (6-X-Trp) into di-halogenated indigoid dyes such as tyrian purple. pTALEsp2 No part name specified with partinfo tag. is an constitutive promoter suited to all strains of E. coli. B0015 BBa_B0015 is a strong terminator. This is a part in a part collection where we enable the production of indigo, tyrian purple, and related dyes from tryptophan in E. coli.
The part collection includes: Parts expressing Fre-SttH to convert Trp to 6-X-Trp. BBa_K4011003 and BBa_K4011012. Parts expressing fusion protein TnaA-FMO to convert 6-X-Trp into indigoid dyes. BBa_K4011004, BBa_K4011005, BBa_K4011013, BBa_K4011014, BBa_K4011015, and BBa_K4011019.
Our part collection can be used to help and inspire future teams to design and perfect different indigoid dye production pathways in E. coli, adding to the collection.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1513
Illegal NheI site found at 1921 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 113
Illegal BamHI site found at 5978
Illegal XhoI site found at 2152
Illegal XhoI site found at 2692 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 747
Illegal AgeI site found at 849
Illegal AgeI site found at 1767
Illegal AgeI site found at 2277
Illegal AgeI site found at 4385 - 1000COMPATIBLE WITH RFC[1000]
None |