Coding

Part:BBa_K4011011

Designed by: Meilun Wu   Group: iGEM21_LINKS_China   (2021-10-21)
Revision as of 11:26, 21 October 2021 by Azahraonng (Talk | contribs)


Ma-CBM3-NT2RepCT-CBM3

Ma-Mut is a mutated protein tag derived from the protein secretion tag (Ma) of mating type alpha Saccharomyces cerevisiae. In yeast cells, proteins with the Ma tag will be secreted out of the cell. This year, we will use Ma-Mut to enable secretion of proteins fused with cellulose binding domain 3 (CBM3) from yeast. CBM3 BBa_K4011000 is an artificial protein derived from Ruminiclostridium thermocellum (Protein Data Bank accession: 1NBC) and will bind to cellulose fibers. NT2RepCT BBa_K3264000 is an artificial spider silk fibroin. This will allow for modification of our bacterial cellulose membrane produced by a co-culture of Komagataeibacter and yeast. We will use Ma_Mut to construct composite parts Ma-sfGFP-CBM3 BBa_K4011010 and Ma-CBM3-NT2RepCT-CBM3 BBa_K4011011.

Other teams can utilize our Ma sequence for S. cerevisiae protein secretion.

Usage and Biology

The alpha-factor preproleader sequence is responsible for protein secretion in mating type alpha Saccharomyces cerevisiae. Mα_Mut is derived from the preproleader sequence and first characterized by Aza et al in 2021, who mutated the sequence to increase protein secretion efficiency.

Natural Mα contains 89 amino acids with three functional regions: a pre-region (19 amino acids), pro-region (64 amino acids), and a spacer (6 amino acids). During S. cerevisiae modification, the preproleader will translocate the protein across the Endoplasmic Reticulum (ER), and the pre-region will be cleaved. Then, the pro-region will direct the protein to the Golgi Apparutus for final modifications before release into media.

CBM3s allow for fused proteins to be bound to cellulose, and NT2RepCT can modify our bacterial cellulose membrane’s properties upon bounding.

Source

Ma comes from S. cerevisiae, NT2RepCT is an artificial spidroin sequence, CBM3 comes from Ruminiclostridium thermocellum.

Design Considerations

1. The following mutations are added in Ma_Mut: A9D, A20T, Q32H, F48S, and G62R.

2. All codons were optimized for S. cerevisiae based on S. cerevisiae codon bias.

3. Linker EAEAFGS used between Ma and first CBM3, flexible linker GSGGGS used between first CBM3 and NT2RepCT, flexible linker GGGGS used between NT2RepCT and second CBM3.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 576
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 948
    Illegal AgeI site found at 2359
    Illegal AgeI site found at 2403
    Illegal AgeI site found at 2575
    Illegal AgeI site found at 2641
    Illegal AgeI site found at 3739
    Illegal AgeI site found at 3783
    Illegal AgeI site found at 3955
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 424


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