Coding

Part:BBa_K4011011

Designed by: Meilun Wu   Group: iGEM21_LINKS_China   (2021-10-21)
Revision as of 11:24, 21 October 2021 by Registry (Talk | contribs)

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Ma-CBM3-NT2RepCT-CBM3

Ma-Mut is a mutated protein tag derived from the protein secretion tag (Ma) of mating type alpha Saccharomyces cerevisiae. In yeast cells, proteins with the Mα tag will be secreted out of the cell. This year, we will use Mα-Mut to enable secretion of proteins fused with cellulose binding domain 3 (CBM3) from yeast. CBM3 BBa_K4011000 is an artificial protein derived from Ruminiclostridium thermocellum (Protein Data Bank accession: 1NBC) and will bind to cellulose fibers. NT2RepCT BBa_K3264000 is an artificial spider silk fibroin. This will allow for modification of our bacterial cellulose membrane produced by a co-culture of Komagataeibacter and yeast. We will use Ma_Mut to construct composite parts Mα-sfGFP-CBM3 BBa_K4011010 and Mα-CBM3-NT2RepCT-CBM3 BBa_K4011011.

Other teams can utilize our Ma sequence for S. cerevisiae protein secretion.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 576
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 948
    Illegal AgeI site found at 2359
    Illegal AgeI site found at 2403
    Illegal AgeI site found at 2575
    Illegal AgeI site found at 2641
    Illegal AgeI site found at 3739
    Illegal AgeI site found at 3783
    Illegal AgeI site found at 3955
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 424


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