Regulatory

Part:BBa_K239000:Design

Designed by: Axel Nystrom   Group: iGEM09_UCL_London   (2009-06-20)
Revision as of 16:03, 20 June 2009 by Registry (Talk | contribs)

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DegP promoter, activated by Phosporylated CpxR and SigmaE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Detect and remove restriction sites: Xba1, EcoRI, Pst1, Spe1. Some uncertanty about where to start and stop in the sequece. It starts 3 bp before the first CpxR binding site and ends 6 pb after the -10 box.


Source

Genomic sequence from biocyc, Escherichia coli K-12. Sequence modified to remove Spe1 restriction site.

References