Part:BBa_K4011009

CBM2-NT2RepCT-CBM2

CBMs are artificial proteins derived from natural proteins with cellulose-binding functions, such as cellulase. There are three types of CBMs, which are CBMs, CBM1, CBM2, and CBM3. CBM1 is the smallest, whilst CBM3 is the biggest. By fusing CBMs to functionalization proteins, we can achieve modification/functionalization of our bacterial cellulose membrane. CBM3-NT2RepCT-CBM3 is constructed from CBM3 BBa_K4011000 and artificial spider fibroin NT2RepCT BBa_K3264000. This is part in a part collection where we characterize bacterial cellulose modification methods and constructs using CBMs.

The part collection includes: Cellulose binding matrixes BBa_K4011000 and BBa_K4011001. CBMs fused with spider silk fibroin BBa_K4011008 and BBa_K4011009. Fused proteins capable of expression and secretion in S. cerevisiae BBa_K4011010 and BBa_K4011011.

This part collection can help and inspire other teams we are trying to achieve modification of cellulose membranes using different modification/functionalization proteins.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 52
  Illegal AgeI site found at 96
  Illegal AgeI site found at 268
  Illegal AgeI site found at 334
  Illegal AgeI site found at 1444
  Illegal AgeI site found at 1488
  Illegal AgeI site found at 1660
  
• 1000
  COMPATIBLE WITH RFC[1000]