Composite

Part:BBa_K3720003

Designed by: Kazuma Tsurumaki   Group: iGEM21_Qdai   (2021-10-01)
Revision as of 10:50, 21 October 2021 by Darjeeling (Talk | contribs)


Firefly luciferase with pCooM, RBS (weak), and double terminator

When used in combination with CooA, it is possible to create a light emitting mechanism that reacts with carbon monoxide. Culture pBEST incorporating this part in LD medium and transfer 20㎕ to a 1.5-ml tube. Then, 0.5㎕ of D-luciferin was added, CO was injected, and luminescence was measured. Quantitative measurements were not possible because the laboratory facilities did not allow us to prepare a luminometer. As shown in the photo, the pipette which carbon monoxide was injected showed a change in color. Left: LB medium+luciferin Right: LB medium+luciferin+CO gas

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 900


[edit]
Categories
//chassis/prokaryote/ecoli
//classic/reporter
//function/reporter/light
Parameters
chassisE. coli K12
colorGreen
controlCooA
device_typereporter
directionForward
outputluciferase