Part:BBa_K3711062
Panb1-α factor-ACC-AOX1 Terminator
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1902
Illegal NheI site found at 2400
Illegal NheI site found at 2442 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 124
Illegal XhoI site found at 649 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1360
Illegal NgoMIV site found at 2120 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1022
Illegal BsaI.rc site found at 2020
Illegal BsaI.rc site found at 2324
Description
This is a composite part for extracellular expression of Acetyl CoA carboxylase. ACC is expressed and participates in the production from acetyl CoA to malonyl coenzyme. Panb1 is a constitutive promoter in yeast, which is expressed under anaerobic conditions, while under aerobic conditions, Panb1, as a repression target of ROX1, is inhibited. When Panb1 initiates the expression, the signal peptide, α-factor, is used to express ACC outside of the cell.
Usage and Biology
Acetyl-CoA carboxylase (ACC) is a biotin enzyme that can catalyze the reaction of "acetyl-CoA+ATP+HCO3→malonyl-CoA+ADP+Pi". It exists widely in nature. ACC is a rate-limiting enzyme for ab initio synthesis of fatty acids, which catalyzes acetyl-CoA to malonyl-CoA, which eventually forms C16 acyl-CoA. ACC can be divided into multi-subunit ACC and multi-functional ACC. Polysubunit ACC exists in plants and bacteria and consists of four subunits, namely, biotin carboxylase (BC), biotin carboxyl carrier protein (BCCP) and two subunits of carboxyltransferase (CT), α-CT and β-CT. Multifunctional ACC mostly exists in eukaryotes. ACC has been used in the drug design of obesity, diabetes and plant herbicides, and is also a target gene for some crops.
Molecular cloning
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