Composite
Part:BBa_K3827007:Design
Designed by: Livia Vieira Group: iGEM21_British_Columbia (2021-10-20)
pBAD - luxCDE -lldR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal NheI site found at 4873
Illegal NheI site found at 4896 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3157
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2563
Illegal SapI site found at 961
Illegal SapI.rc site found at 3689
Design Notes
luxCDE sequence is taken from BioBrick BBa_K325909. They are taken together given they form a heterocomplex that functions as a fatty acid reductase (Brodl et al, 2018).
Source
?