Regulatory

Part:BBa_K4083011

Designed by: Arsen Orazbek   Group: iGEM21_NU_Kazakhstan   (2021-10-20)
Revision as of 08:25, 21 October 2021 by Arsenorazbek (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


lacIq reversed

This is a mutated promoter of lacI, and it contributes to a higher level of transcription of lacI repressor [1]. For our RemiDuET project, we had reversed the lacIq promoter in the pRGPDuo2 plasmid [2].

Reference

[1] Cammack, R. (Ed.) (2006). Oxford Dictionary of Biochemistry and Molecular Biology. (2 ed.) Oxford University Press.

[2] Gauttam, R., Mukhopadhyay, A., & Singer, S. W. (2020). Construction of a novel dual-inducible duet-expression system for gene (over)expression in Pseudomonas putida. Plasmid, 110. https://doi.org/10.1016/j.plasmid.2020.102514

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None